BioLiP

Structure of PDB 9nse Chain B

Receptor sequence

>9nseB (length=414) Species: 9913 (Bos taurus) [Search protein sequence]

KFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRKLQTRPSPG
PPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLR
ESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIK
YATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPA
NVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLE
HPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRN
LCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVT
IVDHHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVN
YILSPAFRYQPDPW

3D structure

PDB

9nse Mapping the active site polarity in structures of endothelial nitric oxide synthase heme domain complexed with isothioureas.

Chain

Resolution

2.24 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	C186 R189 W358 E363
Catalytic site (residue number reindexed from 1)	C118 R121 W290 E295
Enzyme Commision number	1.14.13.39: nitric-oxide synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C96 C101	C28 C33
BS02	H4B	B	W447 F462 Q464	W379 F394 Q396
BS03	HEM	B	W180 C186 M341 F355 S356 W358 E363 W449 Y477	W112 C118 M273 F287 S288 W290 E295 W381 Y409
BS04	H4B	B	S104 R367 A448 W449	S36 R299 A380 W381
BS05	ISU	B	P336 W358 E363	P268 W290 E295	MOAD: Ki=0.039uM
BS06	ISU	B	D202 E208 T211 Y212	D134 E140 T143 Y144	MOAD: Ki=0.039uM

Gene Ontology

	Molecular Function
GO:0004517	nitric-oxide synthase activity

	Biological Process
GO:0006809	nitric oxide biosynthetic process

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Molecular Function

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Biological Process

PDB	RCSB:9nse, PDBe:9nse, PDBj:9nse
PDBsum	9nse
PubMed	11051558
UniProt	P29473\|NOS3_BOVIN Nitric oxide synthase 3 (Gene Name=NOS3)