BioLiP

Structure of PDB 3n65 Chain B

Receptor sequence

>3n65B (length=411) Species: 10116 (Rattus norvegicus) [Search protein sequence]

RFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPVRTKDQLFPL
AKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKH
AWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRS
AITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQ
QGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDL
GLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNHRYNIL
EEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATES
FIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQ
PDPWNTHVWKG

3D structure

PDB

3n65 Role of zinc in isoform-selective inhibitor binding to neuronal nitric oxide synthase .

Chain

Resolution

1.8 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	C415 R418 W587 E592
Catalytic site (residue number reindexed from 1)	C108 R111 W280 E285
Enzyme Commision number	1.14.13.39: nitric-oxide synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	H4B	B	F691 H692 Q693 E694	F384 H385 Q386 E387
BS02	ZN	B	C326 C331	C28 C33
BS03	XFJ	B	W306 F691 H692	W8 F384 H385	BindingDB: Ki=25nM
BS04	HEM	B	W409 C415 M570 F584 W587 E592 R596 F704	W102 C108 M263 F277 W280 E285 R289 F397
BS05	H4B	B	S334 V677 W678	S36 V370 W371
BS06	MTL	B	A497 N498 Q500 F501 N569 D709 W711	A190 N191 Q193 F194 N262 D402 W404
BS07	XFJ	B	M336 V567 F584 W587 E592 R596	M38 V260 F277 W280 E285 R289	BindingDB: Ki=25nM
BS08	XFJ	B	R596 D600 V677 W678	R289 D293 V370 W371	BindingDB: Ki=25nM
BS09	CL	B	R596 D600	R289 D293

Gene Ontology

	Molecular Function
GO:0004517	nitric-oxide synthase activity

	Biological Process
GO:0006809	nitric oxide biosynthetic process

View graph for
Molecular Function

View graph for
Biological Process

PDB	RCSB:3n65, PDBe:3n65, PDBj:3n65
PDBsum	3n65
PubMed	21138269
UniProt	P29476\|NOS1_RAT Nitric oxide synthase 1 (Gene Name=Nos1)