Structure of PDB 1eop Chain B

Receptor sequence

>1eopB (length=241) Species: 562 (Escherichia coli) [Search protein sequence]

SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATSLKTYNIN
ELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKG
IFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK

3D structure

PDB

1eop Crystallographic snapshots along a protein-induced DNA-bending pathway.

Chain

Resolution

2.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Q69 S183 G184 N185	Q68 S179 G180 N181
BS02	dna	B	T37 N70 K92 T93 Y95 T111 K119 R140 T186 N188	T36 N69 K91 T92 Y94 T110 K118 R139 T182 N184

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0004519	endonuclease activity
GO:0009036	type II site-specific deoxyribonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0009307	DNA restriction-modification system

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Molecular Function

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Biological Process

External links

PDB	RCSB:1eop, PDBe:1eop, PDBj:1eop
PDBsum	1eop
PubMed	10801972
UniProt	P04390\|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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