BioLiP

Structure of PDB 2ixh Chain A

Receptor sequence

>2ixhA (length=184) Species: 287 (Pseudomonas aeruginosa) [Search protein sequence]

SMAMKATRLAIPDVILFEPRVFGDDRGFFFESYNQRAFEEACGHPVSFVQ
DNHSRSARGVLRGLHYQIRQAQGKLVRATLGEVFDVAVDLRRGSPTFGQW
VGERLSAENKRQMWIPAGFAHGFVVLSEYAEFLYKTTDFWAPEHERCIVW
NDPELKIDWPLQDAPLLSEKDRQGKAFADADCFP

3D structure

PDB

2ixh RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation.

Chain

Resolution

2.0 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H65 K74 Y134 D171
Catalytic site (residue number reindexed from 1)	H65 K74 Y134 D171
Enzyme Commision number	5.1.3.13: dTDP-4-dehydrorhamnose 3,5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TRH	A	R62 H65 K74 H121 Y134 W140 K170	R62 H65 K74 H121 Y134 W140 K170	MOAD: Ka=11600M^-1

Gene Ontology

	Molecular Function
GO:0008830	dTDP-4-dehydrorhamnose 3,5-epimerase activity
GO:0016853	isomerase activity

	Biological Process
GO:0009103	lipopolysaccharide biosynthetic process
GO:0009244	lipopolysaccharide core region biosynthetic process
GO:0019305	dTDP-rhamnose biosynthetic process
GO:0045226	extracellular polysaccharide biosynthetic process

	Cellular Component
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

PDB	RCSB:2ixh, PDBe:2ixh, PDBj:2ixh
PDBsum	2ixh
PubMed	17046787
UniProt	Q9HU21\|RMLC_PSEAE dTDP-4-dehydrorhamnose 3,5-epimerase (Gene Name=rmlC)