Structure of PDB 2cbj Chain A

Receptor sequence

>2cbjA (length=585) Species: 1502 (Clostridium perfringens) [Search protein sequence]

QVLVPNLNPTPENLEVVGDGFKITSSINLVGEEEADENAVNALREFLTAN
NIEINSENDPNSTTLIIGEVDDDIPELDEALNGTTAENLKEEGYALVSND
GKIAIEGKDGDGTFYGVQTFKQLVKESNIPEVNITDYPTVSARGIVEGFY
GTPWTHQDRLDQIKFYGENKLNTYIYAPKDDPYHREKWREPYPESEMQRM
QELINASAENKVDFVFGISPGIDIRFDGDAGEEDFNHLITKAESLYDMGV
RSFAIYWDDIQDKSAAKHAQVLNRFNEEFVKAKGDVKPLITVPTEYDTGA
MVSNGQPRAYTRIFAETVDPSIEVMWTGPGVVTNEIPLSDAQLISGIYNR
NMAVWWNYPVTDYFKGKLALGPMHGLDKGLNQYVDFFTVNPMEHAELSKI
SIHTAADYSWNMDNYDYDKAWNRAIDMLYGDLAEDMKVFANHSTRMDNKT
WAKSGREDAPELRAKMDELWNKLSSKEDASALIEELYGEFARMEEACNNL
KANLPEVALEECSRQLDELITLAQGDKASLDMIVAQLNEDTEAYESAKEI
AQNKLNTALSSFAVISEKVAQSFIQEALSFDLTLI

3D structure

PDB

2cbj Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis.

Chain

Resolution

2.35 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.2.1.169: protein O-GlcNAcase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	OAN	A	G187 K218 D297 D298 Y335 V370 W394 N396 D401 Y402 W490	G148 K179 D258 D259 Y296 V331 W355 N357 D362 Y363 W451	MOAD: Ki=5.4nM PDBbind-CN: -logKd/Ki=8.27,Ki=5.4nM
BS02	OAN	A	L512 S513	L473 S474	MOAD: Ki=5.4nM PDBbind-CN: -logKd/Ki=8.27,Ki=5.4nM

External links

PDB	RCSB:2cbj, PDBe:2cbj, PDBj:2cbj
PDBsum	2cbj
PubMed	16541109
UniProt	Q0TR53\|OGA_CLOP1 O-GlcNAcase NagJ (Gene Name=nagJ)

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