Structure of PDB 1cl8 Chain A

Receptor sequence

>1cl8A (length=261) Species: 562 (Escherichia coli) [Search protein sequence]

SQGVIGIFGDYAKAHDLAVGEVSKLVKKALSNEYPQLSFRYRDSIKKTEI
NEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEWRVVLVAEAKHQG
KDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHFPYV
LFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSN
LCINKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVL
GRDLFEQLTSK

3D structure

PDB

1cl8 Iap (Inner-Adenine to Purine): A Cognate EcoRI-DNA Base Analog Complex

Chain

Resolution

1.8 Å

3D
structure

[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Enzyme Commision number

3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	N85 S86 S87 K89 H114 Q115 G116 K117 G129 K130 M137 A138 G140 N141 A142 R145	N69 S70 S71 K73 H98 Q99 G100 K101 G113 K114 M121 A122 G124 N125 A126 R129

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003677	DNA binding
GO:0004519	endonuclease activity
GO:0009036	type II site-specific deoxyribonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0009307	DNA restriction-modification system

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:1cl8, PDBe:1cl8, PDBj:1cl8
PDBsum	1cl8
PubMed
UniProt	P00642\|T2E1_ECOLX Type II restriction enzyme EcoRI (Gene Name=ecoRIR)

[Back to BioLiP]

zhanglab

zhanggroup.org | +65-6601-1241 | Computing 1, 13 Computing Drive, Singapore 117417