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●I-TASSER ●I-TASSER-MTD ●C-I-TASSER ●CR-I-TASSER ●QUARK ●C-QUARK ●LOMETS ●MUSTER ●CEthreader ●SEGMER ●DeepFold ●DeepFoldRNA ●FoldDesign ●COFACTOR ●COACH ●MetaGO ●TripletGO ●IonCom ●FG-MD ●ModRefiner ●REMO ●DEMO ●DEMO-EM ●SPRING ●COTH ●Threpp ●PEPPI ●BSpred ●ANGLOR ●EDock ●BSP-SLIM ●SAXSTER ●FUpred ●ThreaDom ●ThreaDomEx ●EvoDesign ●BindProf ●BindProfX ●SSIPe ●GPCR-I-TASSER ●MAGELLAN ●ResQ ●STRUM ●DAMpred

●TM-score ●TM-align ●US-align ●MM-align ●RNA-align ●NW-align ●LS-align ●EDTSurf ●MVP ●MVP-Fit ●SPICKER ●HAAD ●PSSpred ●3DRobot ●MR-REX ●I-TASSER-MR ●SVMSEQ ●NeBcon ●ResPRE ●TripletRes ●DeepPotential ●WDL-RF ●ATPbind ●DockRMSD ●DeepMSA ●FASPR ●EM-Refiner ●GPU-I-TASSER

●BioLiP ●E. coli ●GLASS ●GPCR-HGmod ●GPCR-RD ●GPCR-EXP ●Tara-3D ●TM-fold ●DECOYS ●POTENTIAL ●RW/RWplus ●EvoEF ●HPSF ●THE-DB ●ADDRESS ●Alpaca-Antibody ●CASP7 ●CASP8 ●CASP9 ●CASP10 ●CASP11 ●CASP12 ●CASP13 ●CASP14

Structure of PDB 5m4j Chain A Binding Site BS02

Receptor Information >5m4j Chain A (length=484) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure] GPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQ RYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHA TWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSG SVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEA HREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLH YGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAV YEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMV QAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQP GMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDV VVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPF

Ligand information Ligand ID PRO InChI InChI=1S/C5H9NO2/c7-5(8)4-2-1-3-6-4/h4,6H,1-3H2,(H,7,8)/t4-/m0/s1 InChIKey ONIBWKKTOPOVIA-BYPYZUCNSA-N SMILES Software SMILES OpenEye OEToolkits 1.5.0 C1C[C@H](NC1)C(=O)O CACTVS 3.341 OC(=O)[C@@H]1CCCN1 CACTVS 3.341 OC(=O)[CH]1CCCN1 OpenEye OEToolkits 1.5.0 C1CC(NC1)C(=O)O ACDLabs 10.04 O=C(O)C1NCCC1 Formula C5 H9 N O2 Name PROLINE ChEMBL CHEMBL54922 DrugBank DB00172 ZINC ZINC000000895360 PDB chain 5m4j Chain A Residue 504 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]

Receptor-Ligand Complex Structure Global view Local view Structure summary [Spin on] [Spin off] [Reset] [High quality] [Low quality] [White background] [Black background] [Spin on] [Spin off] [Reset] [High quality] [Low quality] [White background] [Black background] PDB 5m4j Substrate specificity and reaction mechanism of human prolidase. Resolution 1.55 Å Binding residue (original residue number in PDB) H255 H377 R398 E412 R450 Binding residue (residue number reindexed from 1) H250 H372 R393 E407 R445 Annotation score 1

Enzymatic activity Catalytic site (original residue number in PDB) D60 H255 D276 D287 H366 H370 H377 E412 Y416 R450 E452 Catalytic site (residue number reindexed from 1) D55 H250 D271 D282 H361 H365 H372 E407 Y411 R445 E447 Enzyme Commision number 3.4.13.9: Xaa-Pro dipeptidase.

Gene Ontology Molecular Function GO:0004181 metallocarboxypeptidase activity GO:0005515 protein binding GO:0008233 peptidase activity GO:0008237 metallopeptidase activity GO:0016805 dipeptidase activity GO:0030145 manganese ion binding GO:0046872 metal ion binding GO:0070006 metalloaminopeptidase activity GO:0102009 proline dipeptidase activity

	Biological Process
GO:0006508	proteolysis
GO:0006520	amino acid metabolic process
GO:0030574	collagen catabolic process
GO:0043069	negative regulation of programmed cell death

	Cellular Component
GO:0070062	extracellular exosome

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